Rapid Vienna struck in less time than it takes Usain Bolt to run to the end of his garden.
They then displayed barnacle qualities to hold on to their lead - leaving the Midlanders looking to win by two goals in a week's time to make it to the Europa League group stage.
Villa boss O'Neill said: "It was very disappointing to concede so quickly. But I was delighted with the response."
Christopher Drazan crossed for the unmarked Nikica Jelavic, who headed Rapid's quickfire winner.
TEAM RATINGS
VILLA: Guzan 7, Beye 5, Cuellar 6, Davies 6 (Lowry 4), Shorey 5, Milner 6, Reo-Coker 6, Gardner 5 (Agbonlahor 5), Sidwell 6, Young 6, Heskey 5. Subs not used: Friedel, Albrighton, Delfouneso, Delph, Petrov. Booked: Sidwell.
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